Abstract
Replication of human immunodeficiency virus type 1 (HIV-1) is restricted to CD4-expressing primate cells. This tropism may be due partly to the absence from nonprimate cells of a species-specific factor which has an accessory role to CD4 during virus penetration. In this study we describe a rat B lymphocyte cell line in which there is efficient CD4-dependent entry of HIV-1. However, this cell line has a block to productive infection of HIV-1 at a stage between reverse transcription and integration. Our results demonstrate that the putative accessory factor for HIV-1 penetration is not restricted to primate cells and that there is a novel, uncharacterized cell-virus interaction at a stage between penetration and integration.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / pharmacology
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Antigens, CD / biosynthesis
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Antigens, CD / physiology
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B-Lymphocytes / virology*
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Base Sequence
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CD4 Antigens / biosynthesis
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CD4 Antigens / physiology
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Cell Line
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Cricetinae
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DNA Primers
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DNA, Viral / biosynthesis*
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HIV Reverse Transcriptase
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HIV-1 / genetics
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HIV-1 / physiology*
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HeLa Cells
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Humans
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Kidney
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Molecular Sequence Data
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Multiple Myeloma
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Polymerase Chain Reaction
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RNA-Directed DNA Polymerase / metabolism
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Rats
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Virus Integration*
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Virus Replication* / drug effects
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Zidovudine / pharmacology
Substances
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Antibodies, Monoclonal
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Antigens, CD
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CD4 Antigens
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DNA Primers
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DNA, Viral
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Zidovudine
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HIV Reverse Transcriptase
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RNA-Directed DNA Polymerase