Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection

E V Agapov; I A Razumov; I V Frolov; A A Kolykhalov; S V Netesov; V B Loktev

doi:10.1007/BF01309462

Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection

Arch Virol. 1994;139(1-2):173-81. doi: 10.1007/BF01309462.

Authors

E V Agapov¹, I A Razumov, I V Frolov, A A Kolykhalov, S V Netesov, V B Loktev

Affiliation

¹ Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Russia.

PMID: 7529989
DOI: 10.1007/BF01309462

Abstract

Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Capsid / genetics
Capsid / immunology*
Chlorocebus aethiops
Codon / genetics
Encephalitis Virus, Venezuelan Equine / genetics
Encephalitis Virus, Venezuelan Equine / immunology*
Encephalitis Virus, Venezuelan Equine / pathogenicity*
Epitopes / analysis*
Genetic Variation
Mice
Molecular Sequence Data
Neutralization Tests
Point Mutation*
Vero Cells
Virulence

Substances

Antibodies, Monoclonal
Codon
Epitopes