Multiple, diverse senile plaque-associated proteins are ligands of an apolipoprotein E receptor, the alpha 2-macroglobulin receptor/low-density-lipoprotein receptor-related protein

Ann Neurol. 1995 Feb;37(2):211-7. doi: 10.1002/ana.410370212.

Abstract

Both apolipoprotein E and its receptor, the low-density-lipoprotein receptor-related protein (LRP), are associated with senile plaques in Alzheimer's disease. We examined the relationship of other LRP-related molecules to senile plaques. LRP is a multifunctional receptor that binds and rapidly internalizes at least seven ligands: apolipoprotein E, activated alpha 2-macroglobulin, tissue and urokinase-type plasminogen activators, plasminogen activator inhibitor-1, lipoprotein lipase, and lactoferrin. Using immunohistochemistry, we showed that all of these ligands, representing a diverse group of otherwise apparently unrelated proteins, accumulate on senile plaques. We also studied expression of the receptor-associated protein, a physiological inhibitor of LRP, in the hippocampal formation from normal subjects and Alzheimer's disease patients. Receptor-associated protein colocalizes with LRP on neuronal soma, but not on neuronal processes or reactive astrocytes. It is not present on senile plaques. These results suggest that senile plaque-associated LRP can bind its ligands, but clearance of these compounds may be impaired in the vicinity of senile plaques.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alzheimer Disease / pathology*
  • Apolipoproteins E / analysis
  • Apolipoproteins E / metabolism
  • Brain / pathology*
  • Hippocampus / chemistry
  • Hippocampus / metabolism
  • Humans
  • Ligands
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Receptors, Immunologic / analysis*
  • Receptors, Immunologic / metabolism
  • Receptors, LDL / analysis*
  • Receptors, LDL / metabolism
  • Reference Values
  • alpha-Macroglobulins / analysis*
  • alpha-Macroglobulins / metabolism

Substances

  • Apolipoproteins E
  • Ligands
  • Low Density Lipoprotein Receptor-Related Protein-1
  • Receptors, Immunologic
  • Receptors, LDL
  • alpha-Macroglobulins