Abstract
cDNAs encoding the receptors for murine G-CSF, M-CSF or human GM-CSF were inserted into the murine hemopoietic continuous cell lines Ba/F3 or FDC-P1 and sublines selected that were then responsive to proliferative stimulation by these growth factors. When used in microwell assays the Ba/F3 G-CSF receptor-expressing cell line was able to detect 100 pg G-CSF per ml, the Ba/F3 M-CSF receptor-expressing cell line 100-400 pg M-CSF per ml and the FDC-P1 line expressing the alpha- and beta-chains of the human GM-CSF receptor detected 5-10 pg/ml of GM-CSF in test material. These cell lines appear satisfactory for use as selective bioassays for these colony stimulating factors in material potentially containing a mixture of growth factors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Biological Assay / methods*
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Cell Line / chemistry*
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DNA, Complementary / analysis
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Electroporation
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Granulocyte Colony-Stimulating Factor / physiology*
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Granulocyte-Macrophage Colony-Stimulating Factor / physiology*
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Humans
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Macrophage Colony-Stimulating Factor / physiology*
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Mice
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Receptor, Macrophage Colony-Stimulating Factor / analysis
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Receptors, Colony-Stimulating Factor / analysis*
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Receptors, Colony-Stimulating Factor / genetics
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Receptors, Granulocyte Colony-Stimulating Factor / analysis
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / analysis
Substances
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DNA, Complementary
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Receptors, Colony-Stimulating Factor
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Receptors, Granulocyte Colony-Stimulating Factor
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Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
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Granulocyte Colony-Stimulating Factor
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Macrophage Colony-Stimulating Factor
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Granulocyte-Macrophage Colony-Stimulating Factor
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Receptor, Macrophage Colony-Stimulating Factor