Bone marrow biopsy imprints (touch preparations) for assessment of iron stores

Am J Hematol. 1995 Mar;48(3):201-2. doi: 10.1002/ajh.2830480311.

Abstract

Noninvasive evaluation for iron deficiency is compromised in many individuals due to the presence of chronic inflammatory processes and/or malignancy, thus necessitating bone marrow examination for definitive diagnosis. However, bone marrow aspiration is not obtainable or is inadequate for interpretation (dry tap) in some individuals, and decalcified bone marrow biopsies require 24-48 hr to prepare, and may falsely indicate absence of iron. We evaluated the accuracy of bone marrow biopsy imprints (touch preparations) compared with aspirate particle smears for semiquantitation of bone marrow iron stores. Results indicate that Prussian blue-stained bone marrow biopsy imprints accurately reflect the quantity of iron, compared with bone marrow aspirate particle smears, allowing for rapid determination of iron stores in individuals in whom a bone marrow aspirate cannot be obtained.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biopsy
  • Bone Marrow / chemistry*
  • Ferrocyanides
  • Histological Techniques*
  • Humans
  • Iron Deficiencies*
  • Staining and Labeling

Substances

  • Ferrocyanides
  • ferric ferrocyanide