Abstract
Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.
MeSH terms
-
Base Sequence
-
DNA, Antisense / chemical synthesis
-
DNA, Antisense / metabolism
-
DNA, Antisense / pharmacology*
-
DNA, Complementary / biosynthesis
-
Molecular Sequence Data
-
Moloney murine leukemia virus / enzymology
-
Oligodeoxyribonucleotides / chemical synthesis
-
Oligodeoxyribonucleotides / metabolism
-
Oligodeoxyribonucleotides / pharmacology*
-
Polydeoxyribonucleotides / chemical synthesis
-
Polydeoxyribonucleotides / metabolism
-
Polydeoxyribonucleotides / pharmacology*
-
Reverse Transcriptase Inhibitors
-
Ribonuclease H
-
Transcription, Genetic / drug effects*
Substances
-
DNA, Antisense
-
DNA, Complementary
-
Oligodeoxyribonucleotides
-
Polydeoxyribonucleotides
-
Reverse Transcriptase Inhibitors
-
Ribonuclease H