Abstract
Granulocyte colony-stimulating factor(G-CSF), but not granulocyte-macrophage CSF(GM-CSF), induced tyrosine phosphorylation of 95-kDa protein in 13 cases of primary human acute myelogenous leukemic cells. Electrophoretic mobility of 95-kDa phosphoprotein and the protooncogene product p95vav was identical. In addition, p95vav was tyrosine-phosphorylated only in G-CSF-stimulated cells. In contrast to primary leukemic cells, amount of p95vav was under detectable level and G-CSF did not induce tyrosine phosphorylation of 90-100-kDa proteins in human neutrophils. These results indicate specific involvement of vav product in signaling pathway of G-CSF in primary human leukemic cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antibodies
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Blotting, Western
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Cell Cycle Proteins*
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Granulocyte Colony-Stimulating Factor / pharmacology
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Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
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Humans
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Leukemia, Myeloid, Acute
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Molecular Sequence Data
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Peptides / chemical synthesis
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Peptides / immunology
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Phosphoproteins / isolation & purification
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Phosphoproteins / metabolism
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Phosphorylation
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Phosphotyrosine
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-vav
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Proto-Oncogenes* / drug effects
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Recombinant Proteins / pharmacology
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Tumor Cells, Cultured
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Tyrosine / analogs & derivatives*
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Tyrosine / analysis
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Tyrosine / metabolism
Substances
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Antibodies
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Cell Cycle Proteins
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Peptides
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Phosphoproteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-vav
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Recombinant Proteins
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VAV1 protein, human
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Granulocyte Colony-Stimulating Factor
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Phosphotyrosine
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Tyrosine
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Granulocyte-Macrophage Colony-Stimulating Factor