Abstract
YY1 is a zinc finger transcription factor with unusual structural and functional features. In a yeast two-hybrid screen, two cellular proteins, cyclophilin A (CyPA) and FK506-binding protein 12 (FKBP12), interacted with YY1. These interactions are specific and also occur in mammalian cells. Cyclosporin A and FK506 efficiently disrupt the YY1-CyPA and YY1-FKBP12 interactions. Overexpression of human CyPA and FKBP12 have different effects on YY1-regulated transcription with these effects being promoter-dependent. These results suggest that immunophilins may be mediators in the functional role of YY1.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Isomerases / metabolism*
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Carrier Proteins / metabolism*
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Chloramphenicol O-Acetyltransferase / metabolism
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Cyclosporine / pharmacology
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DNA-Binding Proteins / metabolism*
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Endodeoxyribonucleases / metabolism
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Erythroid-Specific DNA-Binding Factors
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HeLa Cells
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Heat-Shock Proteins / metabolism*
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Humans
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Peptidylprolyl Isomerase
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Promoter Regions, Genetic
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Recombinant Fusion Proteins / metabolism
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TATA Box
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Tacrolimus / pharmacology
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Tacrolimus Binding Proteins
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Transcription Factors / metabolism*
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Transcription, Genetic* / drug effects
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Transfection
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YY1 Transcription Factor
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Zinc Fingers
Substances
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Carrier Proteins
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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Heat-Shock Proteins
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Recombinant Fusion Proteins
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Transcription Factors
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YY1 Transcription Factor
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YY1 protein, human
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Cyclosporine
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Chloramphenicol O-Acetyltransferase
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Endodeoxyribonucleases
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Amino Acid Isomerases
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Tacrolimus Binding Proteins
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Peptidylprolyl Isomerase
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Tacrolimus