Application of the polymerase chain reaction to the detection of African horse sickness viruses

S Zientara; C Sailleau; S Moulay; A Wade-Evans; C Cruciere

doi:10.1016/0166-0934(94)00175-g

Application of the polymerase chain reaction to the detection of African horse sickness viruses

J Virol Methods. 1995 May;53(1):47-54. doi: 10.1016/0166-0934(94)00175-g.

Authors

S Zientara¹, C Sailleau, S Moulay, A Wade-Evans, C Cruciere

Affiliation

¹ CNEVA-Laboratoire Central de Recherches Vétérinaires, Maisons-Alfort, France.

PMID: 7543488
DOI: 10.1016/0166-0934(94)00175-g

Abstract

The development of a coupled reverse transcriptase-polymerase chain reaction assay (RT-PCR) is described for the detection of African horse sickness virus (AHSV) double-stranded RNA. Genome segments 7 and 10 were chosen as target templates for primers selected for use in the RT-PCR. Using these AHSV-specific primers all 9 serotypes were detectable. The sensitivity and specificity of the RT-PCR results were compared to those obtained by competition ELISA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

African Horse Sickness / virology*
African Horse Sickness Virus / genetics
African Horse Sickness Virus / isolation & purification*
Animals
Base Sequence
DNA Primers
Enzyme-Linked Immunosorbent Assay / veterinary
Horses
Mice
Molecular Sequence Data
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / veterinary*
RNA, Double-Stranded / analysis
RNA, Viral / analysis
RNA-Directed DNA Polymerase
Sensitivity and Specificity
Spleen / virology

Substances

DNA Primers
RNA, Double-Stranded
RNA, Viral
RNA-Directed DNA Polymerase