Abstract
Rabbit hearts were preconditioned with four 5 min coronary artery occlusions 24 h before 30 min coronary occlusion with 120 min reperfusion. Preconditioning significantly reduced the percentage of myocardium infarcting within the risk zone from 49.1 +/- 4.3% to 31.8 +/- 3.5% (P < 0.05). When the protein kinase C (PKC) inhibitor, chelerythrine, was administered just before preconditioning, the delayed protection against infarction 24 h later was abolished. We conclude that the delayed cytoprotective response associated with ischaemic preconditioning of myocardium is likely to involve the early activation of one or more PKC subtypes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids
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Analysis of Variance
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Animals
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Benzophenanthridines
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Blood Pressure / drug effects
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Cadmium / metabolism
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Cadmium Compounds*
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Coronary Disease / physiopathology
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Disease Models, Animal
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Enzyme Activation / drug effects
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Heart Rate / drug effects
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Male
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Microspheres
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Myocardial Ischemia / drug therapy
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Myocardial Ischemia / enzymology*
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Phenanthridines / pharmacology*
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Phenanthridines / therapeutic use
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / metabolism*
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Rabbits
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Staining and Labeling
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Sulfides*
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Tetrazolium Salts / chemistry
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Zinc / metabolism
Substances
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Alkaloids
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Benzophenanthridines
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Cadmium Compounds
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Phenanthridines
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Sulfides
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Tetrazolium Salts
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Cadmium
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cadmium sulfide
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triphenyltetrazolium
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chelerythrine
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Protein Kinase C
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Zinc