Abstract
Sympathetic neurons from perinatal rat pups extend only a single axon when maintained in culture in the absence of glia and serum. Exposure to recombinant osteogenic protein-1 (OP-1) selectively induces the formation of dendrites that correctly segregate and modify cytoskeletal and membrane proteins and form synaptic contacts of appropriate polarity. OP-1 requires nerve growth factor (NGF) as a cofactor, and, in the presence of optimal concentrations of NGF, OP-1-induced dendritic growth from cultured perinatal neurons is comparable to that observed in situ. Sympathetic neuroblasts that had not formed dendrites in situ also responded to OP-1 in culture, indicating that OP-1 can cause de novo formation as well as regeneration of dendrites. These data imply that specific signals can regulate the development of neuronal shape and polarity.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Animals, Newborn
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins*
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CHO Cells
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Cells, Cultured
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Cricetinae
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Dendrites / physiology*
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Dendrites / ultrastructure
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Dose-Response Relationship, Drug
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Ganglia, Sympathetic / ultrastructure*
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Growth Substances / administration & dosage
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Growth Substances / pharmacology
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Humans
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Nerve Growth Factors / administration & dosage
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Nerve Growth Factors / pharmacology
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Neurons / ultrastructure*
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Proteins / administration & dosage
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Proteins / pharmacology*
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Rats
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Recombinant Proteins / pharmacology
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Synapses / ultrastructure
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Transforming Growth Factor beta*
Substances
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BMP7 protein, human
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Bmp7 protein, rat
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Bone Morphogenetic Protein 7
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Bone Morphogenetic Proteins
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Growth Substances
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Nerve Growth Factors
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Proteins
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Recombinant Proteins
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Transforming Growth Factor beta