Comparison of apo A-I-containing subpopulations of dog plasma and prenodal peripheral lymph: evidence for alteration in subpopulations in the interstitial space

B F Asztalos; C H Sloop; L Wong; P S Roheim

doi:10.1016/0005-2760(93)90254-7

Comparison of apo A-I-containing subpopulations of dog plasma and prenodal peripheral lymph: evidence for alteration in subpopulations in the interstitial space

Biochim Biophys Acta. 1993 Sep 8;1169(3):301-4. doi: 10.1016/0005-2760(93)90254-7.

Authors

B F Asztalos¹, C H Sloop, L Wong, P S Roheim

Affiliation

¹ Department of Physiology, Louisiana State University Medical Center, New Orleans 70112, USA.

PMID: 7548124
DOI: 10.1016/0005-2760(93)90254-7

Abstract

To study in vivo reverse cholesterol transport, dog plasma and lymph apo A-I-containing subpopulations were compared by two-dimensional electrophoresis. Charge and size of subpopulations were similar in plasma and lymph, but the distribution of subpopulations varied considerably. An increase in pre-beta and pre-alpha particles in lymph suggests these changes are a reflection of in vivo reverse cholesterol transport.

Publication types

Comparative Study
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Apolipoprotein A-I / analysis*
Biological Transport
Cholesterol / metabolism
Dogs
Electrophoresis, Gel, Two-Dimensional / methods*
Extracellular Space / chemistry*
Lipoproteins, HDL / blood*
Lipoproteins, HDL / chemistry
Lipoproteins, HDL / isolation & purification
Lymph / chemistry*

Substances

Apolipoprotein A-I
Lipoproteins, HDL
Cholesterol

Grants and funding

HL25596/HL/NHLBI NIH HHS/United States