Abstract
As a marker of macrophage activation, IL-1 alpha was measured after stimulation of murine resident peritoneal macrophages (RPM) with endotoxin-associated protein (EP). Significant IL-1 alpha was produced by EP-stimulated RPM from both C3H/OuJ and C3H/HeJ mouse strains. This EP-mediated IL-1 alpha production was blocked by tyrosine kinase inhibitors including genistein and tyrphostin, suggesting the involvement of a protein tyrosine kinase in the activation of RPM by EP. Immunoblot analysis using antiphosphotyrosine antibody showed that EP induces the tyrosine phosphorylation of a 71 kD protein (p71). The p71 and the spleen tyrosine kinase p72syk found in other cell types share common features including: similar molecular weight, PKC independent tyrosine phosphorylation, and inhibition of phosphorylation by piceatannol. Furthermore, immunoblot analysis using anti-p72syk antibody detected the p72syk kinase in EP-activated RPM. These results suggest that the activation of RPM involves an early tyrosine phosphorylation of p72syk or a p72syk-like protein.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacterial Proteins / pharmacology*
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Endotoxins / pharmacology*
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Enzyme Inhibitors / pharmacology
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Enzyme Precursors / antagonists & inhibitors
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Enzyme Precursors / immunology
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Enzyme Precursors / metabolism*
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Female
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Immunoblotting
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Interferon Type I / pharmacology
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Interleukin-1 / biosynthesis*
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Intracellular Signaling Peptides and Proteins
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Lipid A / pharmacology*
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Macrophage Activation
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Macrophages, Peritoneal / enzymology
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Macrophages, Peritoneal / metabolism*
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Male
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Mice
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Mice, Inbred C3H
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Phosphoproteins / immunology
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Phosphoproteins / metabolism
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Phosphorylation
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Pregnancy Proteins / pharmacology
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Protein Kinase Inhibitors
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Protein-Tyrosine Kinases / antagonists & inhibitors
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Protein-Tyrosine Kinases / immunology
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Protein-Tyrosine Kinases / metabolism*
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Signal Transduction
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Syk Kinase
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Tetradecanoylphorbol Acetate / pharmacology
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Tyrosine / metabolism*
Substances
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Bacterial Proteins
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Endotoxins
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Enzyme Inhibitors
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Enzyme Precursors
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Interferon Type I
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Interleukin-1
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Intracellular Signaling Peptides and Proteins
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Lipid A
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Phosphoproteins
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Pregnancy Proteins
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Protein Kinase Inhibitors
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interferon tau
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lipid A-associated protein, Bacteria
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Tyrosine
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Protein-Tyrosine Kinases
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Syk Kinase
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Syk protein, mouse
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Tetradecanoylphorbol Acetate