House dust mites, Dermatophagoides pteronyssinus (Dp) and Dermatophagoides farinae (Df) are the predominant cause of allergic asthma in our population. To detect the presence of sensitivity to other mites besides Dp and Df in 100 children that live in Mexico City, skin tests were performed in their arms with Multi-test devices and glycerinated extracts. There were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. Dp was positive in 96, Df in 80, Euroglyphus maynei in 41, Chortoglyphus in 22, Blomia tropicalis in 17, Trophagus putrescentiae in 12, Glycphagus in 12, Acarus siro in 7, Lepidoglyphus destructor in 7 and Gophieria in 7. Hypersensitivity to all of th species was common. Even we know about the cross-reactivity between them, there are species-specific allergens that could make some of them important in diagnosis and treatment.