The use of surface immunofluorescence assay (SIFA) in the microbiological diagnosis of Lyme borreliosis: a case report

New Microbiol. 1995 Jul;18(3):307-10.

Abstract

In this report we describe the use of a newly developed immunofluorescence technique performed with living spirochetes to detect serum antibody to B. burgdorferi s.l. in a case of early Lyme borreliosis. The immunofluorescence method used (surface immunofluorescence assay: SIFA) proved useful in the serological evaluation of suspected cases of Lyme disease.

Publication types

  • Case Reports
  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / analysis*
  • Bites and Stings
  • Blotting, Western
  • Borrelia burgdorferi Group / immunology*
  • Child
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fluorescent Antibody Technique*
  • Humans
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin M / biosynthesis
  • Lyme Disease / diagnosis*
  • Lyme Disease / immunology
  • Lyme Disease / microbiology
  • Ticks / genetics
  • Ticks / microbiology
  • Time Factors

Substances

  • Antibodies, Bacterial
  • Immunoglobulin G
  • Immunoglobulin M