Abstract
Rat ascites hepatoma cells (MM1 cells) penetrate through a cultured mesothelial cell monolayer (MCL) in the presence of fetal calf serum (FCS), but scarcely do so in its absence. Inactivation of rhop21 of MM1 cells by ADP-ribosyltransferase C3 resulted in the suppression of this serum effect on the penetration, suggesting that the serum effect was mediated by rhop21. To ascertain this assumption MM1 cells were transfected with an activated (Val14) human rhoA cDNA (Neo/RhoA 1-7). The transfectants penetrated MCL extensively even in the absence of FCS and became largely independent of serum for the penetration. These results suggest that serum-induced invasion by MM1 cells is mainly mediated by rhop21.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP Ribose Transferases / metabolism
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Animals
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Base Sequence
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Blood
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Botulinum Toxins*
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Cattle
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Cell Movement
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Culture Media / chemistry
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Epithelium / pathology
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / physiology*
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Humans
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Liver Neoplasms, Experimental / pathology*
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Molecular Sequence Data
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Neoplasm Invasiveness*
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Point Mutation / genetics
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
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Rats, Inbred Strains
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Transfection
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Tumor Cells, Cultured
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rho GTP-Binding Proteins
Substances
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Culture Media
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RNA, Messenger
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ADP Ribose Transferases
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exoenzyme C3, Clostridium botulinum
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Botulinum Toxins
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GTP-Binding Proteins
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rho GTP-Binding Proteins