Crystallization and preliminary X-ray investigation of recombinant human interleukin 10

Proteins. 1995 Jun;22(2):187-90. doi: 10.1002/prot.340220211.

Abstract

Crystals of recombinant human interleukin 10 have been grown from solutions of ammonium sulfate. The crystals are tetragonal, space group P4(1)2(1)2 or P4(3)2(1)2; the unit cell axes are a = 36.5 A and c = 221.9 A. There is the equivalent of one polypeptide chain in the asymmetric unit. The crystals are stable to X-rays and diffract to at least 2.5 A resolution.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Ammonium Sulfate / chemistry
  • Animals
  • CHO Cells / metabolism
  • Cricetinae
  • Escherichia coli / metabolism
  • Humans
  • Interleukin-10 / chemistry*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • X-Ray Diffraction

Substances

  • Recombinant Proteins
  • Interleukin-10
  • Ammonium Sulfate