Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95

Science. 1995 Sep 22;269(5231):1737-40. doi: 10.1126/science.7569905.

Abstract

The N-methyl-D-aspartate (NMDA) receptor subserves synaptic glutamate-induced transmission and plasticity in central neurons. The yeast two-hybrid system was used to show that the cytoplasmic tails of NMDA receptor subunits interact with a prominent postsynaptic density protein PSD-95. The second PDZ domain in PSD-95 binds to the seven-amino acid, COOH-terminal domain containing the terminal tSXV motif (where S is serine, X is any amino acid, and V is valine) common to NR2 subunits and certain NR1 splice forms. Transcripts encoding PSD-95 are expressed in a pattern similar to that of NMDA receptors, and the NR2B subunit co-localizes with PSD-95 in cultured rat hippocampal neurons. The interaction of these proteins may affect the plasticity of excitatory synapses.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cytoplasm / chemistry
  • Disks Large Homolog 4 Protein
  • Genes, Reporter
  • Hippocampus / metabolism*
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neuronal Plasticity
  • Neurons / metabolism*
  • RNA Splicing
  • Rats
  • Receptors, N-Methyl-D-Aspartate / chemistry
  • Receptors, N-Methyl-D-Aspartate / genetics
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Signal Transduction

Substances

  • Disks Large Homolog 4 Protein
  • Dlg4 protein, rat
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Receptors, N-Methyl-D-Aspartate
  • Recombinant Fusion Proteins
  • postsynaptic density proteins

Associated data

  • SWISSPROT/P02719
  • SWISSPROT/P07174
  • SWISSPROT/P08510
  • SWISSPROT/P08511
  • SWISSPROT/P08909
  • SWISSPROT/P08953
  • SWISSPROT/P10499
  • SWISSPROT/P12526
  • SWISSPROT/P14842
  • SWISSPROT/P15389
  • SWISSPROT/P15390
  • SWISSPROT/P18090
  • SWISSPROT/P25445
  • SWISSPROT/P26591
  • SWISSPROT/P29534
  • SWISSPROT/P30083
  • SWISSPROT/P34082
  • SWISSPROT/P35331
  • SWISSPROT/P35353
  • SWISSPROT/P35439
  • SWISSPROT/Q00960