Abstract
Klebsiella oxytoca mutants resistant to a variety of beta-lactams were obtained in vitro on aztreonam. Constitutive beta-lactamase production was much higher in the mutants than in the susceptible strains (75-fold). The only difference observed in these mutants compared with the susceptible strains were point mutations in the Pribnow box: a transversion (G-->T) in the first base for one mutant or a transition (G-->A) in the fifth base of the -10 consensus sequence for the other three mutants. The transcriptional output of the beta-lactamase gene (blaOXY) from the mutants was significantly higher than that of the blaOXY gene from the susceptible strains.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Base Sequence
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Conserved Sequence*
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DNA, Bacterial / analysis
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Gene Expression Regulation, Bacterial
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Klebsiella / drug effects
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Klebsiella / genetics*
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Klebsiella / metabolism
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Point Mutation*
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Promoter Regions, Genetic
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Transcriptional Activation
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beta-Lactam Resistance
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics*
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beta-Lactams
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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beta-Lactams
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beta-Lactamases
Associated data
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GENBANK/Z30178
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GENBANK/Z30179
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GENBANK/Z30181
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GENBANK/Z30182
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GENBANK/Z30208