Abstract
Studies of HIV-2 infection have shown lower rates of sexual and perinatal transmission and a prolonged incubation period to AIDS as compared to HIV-1. To evaluate the role of genetic variation in HIV pathogenesis, we studied intrapatient variability in the V3 loop of the HIV-2 envelope gene over time in five seropositive individuals. Proviral sequences derived from uncultured PBMC DNA (n = 102) demonstrated an average sequence heterogeneity within a sample of 1.4% (0-4.1%). This was significantly lower than the V3 sequence heterogeneity observed in HIV-1, which can be as high as 6.1%. In HIV-2-seropositive healthy patients the average intrapatient nucleotide variability rate was 0.6% compared to 2.0% in patients with clinical AIDS. The lower rate of variability between HIV-2 and HIV-1 is compatible with differences in transmission and pathogenesis of these two related viruses.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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CD4 Lymphocyte Count
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DNA Primers
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Female
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Follow-Up Studies
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Genetic Variation*
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HIV Envelope Protein gp120 / genetics*
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HIV Infections / blood
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HIV Infections / physiopathology
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HIV Infections / virology*
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HIV-1 / genetics
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HIV-2 / genetics*
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HIV-2 / isolation & purification
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Humans
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Male
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Molecular Sequence Data
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Peptide Fragments / genetics*
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Polymerase Chain Reaction
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Sequence Homology, Amino Acid
Substances
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DNA Primers
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HIV Envelope Protein gp120
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HIV envelope protein gp120 (305-321)
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Peptide Fragments
Associated data
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GENBANK/U24287
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GENBANK/U24288
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GENBANK/U24289
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GENBANK/U24290
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GENBANK/U24291
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GENBANK/U24292
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GENBANK/U24293
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GENBANK/U24294
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GENBANK/U24295
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GENBANK/U24296
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GENBANK/U24297
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GENBANK/U24298
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GENBANK/U24299
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GENBANK/U24300
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GENBANK/U24301
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GENBANK/U24302
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GENBANK/U24303
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GENBANK/U24304
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GENBANK/U24305
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GENBANK/U24306
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GENBANK/U24307
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GENBANK/U24308
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GENBANK/U24309
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GENBANK/U24310
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GENBANK/U24311
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GENBANK/U24312
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GENBANK/U24313
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GENBANK/U24314
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GENBANK/U24315
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GENBANK/U24316