Abstract
The effect of insulin-like growth factor II (IGF-II) on tumour development in the mouse mammary gland was studied. To promote extra IGF-II expression in the mammary gland, sheep beta-lactoglobulin regulatory elements were attached to the coding regions of the mouse Igf-2 gene and injected into the pronuclei of mouse zygotes. Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland. Tumours from two of the lines grew after transplantation to both male and female hosts. Primary tumours contained stromal and epithelial regions, but the tumours were dominated by mammary adenocarcinoma after transplantation. The tumours expressed high levels of Igf-2 mRNA transcribed from the integrated transgenes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics*
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Adenocarcinoma / physiopathology
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Animals
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Cell Transformation, Neoplastic / genetics*
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Crosses, Genetic
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Female
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Gene Expression Regulation
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Insulin-Like Growth Factor II / biosynthesis
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Insulin-Like Growth Factor II / genetics*
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Insulin-Like Growth Factor II / physiology
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Lactoglobulins / genetics
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Male
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Mammary Glands, Animal / metabolism
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Mammary Glands, Animal / pathology
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Mammary Neoplasms, Experimental / genetics*
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Mammary Neoplasms, Experimental / physiopathology
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Mice, Transgenic
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics*
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Regulatory Sequences, Nucleic Acid
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Sheep / genetics
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Transgenes*
Substances
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Lactoglobulins
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Neoplasm Proteins
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Insulin-Like Growth Factor II