Abstract
In an attempt to determine whether immunization of healthy HIV-1 seronegative individuals with a soluble gp160 candidate vaccine could induce an anti-HIV specific immune response, volunteers were immunized by two injections of a water-in-oil emulsion containing a mixture of gp160 antigen together with selected peptides. Following immunization, lymphocytes were collected and stimulated in vitro with autologous HIV-1-infected cells. The results showed that immunization with soluble HIV-1 envelope was able to generate CD3+ CD8+ CTLs directed to gp160 antigen. The CTL response was restricted to class I molecule HLA-A2. The CTL response was comparable to that elicited by immunization with HIV-1-envelope recombinant vaccinia virus.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
AIDS Vaccines / immunology
-
Amino Acid Sequence
-
CD3 Complex / biosynthesis
-
CD4-Positive T-Lymphocytes / immunology
-
Cell Line, Transformed
-
Cell Transformation, Viral
-
Cytotoxicity Tests, Immunologic
-
Gene Products, env / immunology*
-
HIV Envelope Protein gp160
-
Humans
-
Lymphocyte Culture Test, Mixed
-
Molecular Sequence Data
-
Peptide Fragments / immunology
-
Protein Precursors / immunology*
-
T-Lymphocytes, Cytotoxic / cytology
-
T-Lymphocytes, Cytotoxic / immunology*
-
Vaccination
-
Vaccines, Synthetic / administration & dosage
-
Vaccines, Synthetic / immunology
-
Vaccinia virus / immunology
Substances
-
AIDS Vaccines
-
CD3 Complex
-
Gene Products, env
-
HIV Envelope Protein gp160
-
Peptide Fragments
-
Protein Precursors
-
Vaccines, Synthetic