Abstract
We cloned a Bacillus subtilis gene (srb) encoding a homologue of the mammalian signal recognition particle receptor alpha-subunit (SR alpha). The gene is 987 bp in length and encodes a 329-amino acid protein. The deduced amino acid sequence of the protein shared 26.6, 36.2 and 49.7% identity with those of mammalian SR alpha, archaebacterial DP alpha and Escherichia coli FtsY, respectively. The protein contains three conserved GTP-binding elements like the other three SRP receptor proteins, though the N-terminal portion of the putative B. subtilis protein was shorter than the others. Secondary structure prediction showed than an amphipathic alpha-helix is positioned in the N-terminal region. A defect in srb inhibited cell growth and protein translocation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / genetics*
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Bacillus subtilis / growth & development
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Biological Transport / genetics
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Cloning, Molecular
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Conserved Sequence
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Genes, Bacterial / genetics*
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Guanosine Triphosphate / metabolism
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Molecular Sequence Data
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Protein Structure, Secondary
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Receptors, Cytoplasmic and Nuclear / chemistry
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Peptide / chemistry
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Receptors, Peptide / genetics*
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Sequence Homology, Amino Acid
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Sequence Homology, Nucleic Acid
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Signal Recognition Particle / metabolism
Substances
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Bacterial Proteins
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Receptors, Cytoplasmic and Nuclear
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Receptors, Peptide
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Signal Recognition Particle
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signal peptide receptor
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srb protein, Bacillus subtilis
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Guanosine Triphosphate