Abstract
We have made a monoclonal anti-CD44 antibody which is able to activate the leukocyte integrin CD11a/CD18. Activated T cells strongly aggregated, and the aggregation was shown to be intercellular adhesion molecule (ICAM)-1 (CD54) and ICAM-2 (CD102) dependent. Using purified ICAM coated on plastic, only binding to ICAM-1 was increased by the CD44 antibody, whereas activation by phorbol ester increased binding to both ICAM-1 and ICAM-3. The binding to ICAM-2 was not affected by either treatment. These findings show that the CD11a/CD18 integrin can be activated in a ligand-specific manner by engagement of CD44.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Antigens, CD / immunology*
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Antigens, Differentiation*
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CD11 Antigens / immunology*
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Cell Adhesion Molecules / immunology*
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Cell Aggregation / immunology
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Cells, Cultured
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Humans
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Hyaluronan Receptors / immunology*
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Intercellular Adhesion Molecule-1 / immunology*
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Lymphocyte Activation / immunology
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T-Lymphocytes / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation
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CD11 Antigens
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Cell Adhesion Molecules
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Hyaluronan Receptors
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ICAM2 protein, human
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ICAM3 protein, human
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Intercellular Adhesion Molecule-1