Cloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen

Gene. 1995 Oct 27;164(2):255-9. doi: 10.1016/0378-1119(95)00527-d.

Abstract

We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics*
  • Allergens / immunology
  • Amino Acid Sequence
  • Cloning, Molecular
  • Cross Reactions
  • DNA, Complementary
  • Gene Expression
  • Genes, Plant*
  • Molecular Sequence Data
  • Multigene Family
  • Oryza / genetics*
  • Plant Leaves
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Plant Proteins / immunology
  • Plant Roots
  • Pollen
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Species Specificity

Substances

  • Allergens
  • DNA, Complementary
  • Ory s I protein, Oryza sativa
  • Plant Proteins

Associated data

  • GENBANK/U31771