Abstract
In the presence of sodium nitrite and sodium hydrosulfite, the produced NO has been confirmed by the detection of bleomycin-Fe(II)-NO adduct complex. Nitric oxide clearly induces DNA form change of form I-->forms II & III. Of special interest is the fact that (1) NO attacks guanine bases as the most preferential cleaving sites and (2) the frequency of the NO-induced cutting decreases in the order G > C > A > T. The preferred cleavage at GC sequences by NO may correlate to predominant mutagenicity at GC sites by nitric oxide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA / chemistry*
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DNA / drug effects
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DNA Adducts
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DNA Damage*
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DNA, Superhelical / chemistry
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DNA, Superhelical / drug effects
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Electron Spin Resonance Spectroscopy
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Molecular Sequence Data
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Mutagens / chemistry*
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Mutagens / pharmacology
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Nitric Oxide / chemistry*
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Nitric Oxide / pharmacology
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Nucleic Acid Conformation
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Sequence Analysis, DNA
Substances
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DNA Adducts
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DNA, Superhelical
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Mutagens
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Nitric Oxide
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DNA
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Deoxyribonucleases, Type II Site-Specific