Abstract
A 2.4 kilobase cDNA clone of human sulfite oxidase was isolated from a human liver cDNA library in lambda gt10. Comparison of three sulfite oxidase sequences to several plant and fungal nitrate reductase sequences reveals a single conserved cysteine with highly conserved flanking sequences. The conserved cysteine is postulated to be a ligand of molybdenum in sulfite oxidase and nitrate reductase.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Conserved Sequence
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DNA, Complementary / isolation & purification*
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Humans
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Liver / enzymology*
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Molecular Sequence Data
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Oxidoreductases Acting on Sulfur Group Donors / biosynthesis
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Oxidoreductases Acting on Sulfur Group Donors / deficiency
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Oxidoreductases Acting on Sulfur Group Donors / genetics*
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Recombinant Proteins / biosynthesis
Substances
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DNA, Complementary
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Recombinant Proteins
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Oxidoreductases Acting on Sulfur Group Donors