Rare occurrence of p53 and ras gene mutations in preneoplastic and neoplastic mouse endometrial lesions induced by N-methyl-N-nitrosourea and 17 beta-estradiol

Cancer Lett. 1995 Jun 8;92(2):223-7. doi: 10.1016/0304-3835(95)03782-r.

Abstract

To examine K-, H-, or N-ras and p53 gene mutations in mouse endometrial carcinogenesis induced by N-methyl-N-nitrosourea and 17 beta-estradiol, we performed polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) in 13 adenocarcinomas and 11 other preneoplastic lesions. A significant shifted band in exon 5 of p53 using PCR-SSCP was detected in one of 13 adenocarcinomas. Direct sequencing showed that the mutation was TCA-to-TGA (Ser-to-End) transition. These results suggest that ras gene mutations were not related to carcinogenesis and inactivation of p53 may occur with low frequency during the mouse endometrial carcinogenesis in this model.

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / pathology
  • Animals
  • Base Sequence
  • Endometrial Neoplasms / chemically induced*
  • Endometrial Neoplasms / genetics*
  • Endometrial Neoplasms / pathology
  • Endometrium / pathology
  • Estradiol*
  • Exons
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genes, p53*
  • Genes, ras*
  • Hyperplasia / genetics
  • Hyperplasia / pathology
  • Methylnitrosourea*
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Mutation*
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Precancerous Conditions / chemically induced*
  • Precancerous Conditions / genetics*
  • Precancerous Conditions / pathology
  • Tumor Cells, Cultured

Substances

  • Estradiol
  • Methylnitrosourea