We purified profilin from bovine brain and were able to separate the two isoforms present in this tissue. Since functional characteristics for profilin II are lacking, we assayed the actin, the phosphatidylinositol 4,5-bisphosphate and the poly(L-proline) binding properties of this isoform. Profilin II binds actin with a similar affinity to that of profilin I, although it inhibits actin polymerization more strongly than profilin I under non-equilibrium conditions. Profilin II also binds the anionic phospholipid phosphatidylinositol 4,5-bisphosphate. Profilin II binds to poly(L-proline) more strongly than does profilin I; this is especially evident at more acidic pH values. This difference is explained by an amino acid exchange in the carboxy-terminal part of the protein which has been implicated in poly(L-proline) binding [Björkegren, C., Rozycki, M., Schutt, C., Lindberg, U. & Karlsson, R. (1993) FEBS Lett. 333, 123-126; Metzler, W., Bell, A., Ernst, E., Lavoie, T. & Mueller, L. (1994) J. Biol. Chem. 369, 4620-4625].