Abstract
Autonomously replicating sequence binding factor 1 (ABF1) has been implicated in the control of a variety of gene expressions in Saccharomyces cerevisiae. In this paper evidence is presented that ABF1 is involved in the glucose-dependent expression of the TDH3 gene which encodes glyceraldehyde-3-phosphate dehydrogenase. ABF1 binds to consensus sites located between -420 and -250, and between +77 and +200, and acts as a transactivator in an orientation-independent manner on both upstream and downstream sites. TDH3-lacZ fusions having an ABF1 consensus motif showed glucose-dependent expression of TDH3, whereas in the abf1 mutant strain JCA35 glucose-dependent expression disappeared. These findings suggest that ABF1 functions as a glucose-dependent transactivator for the expression of the TDH3 gene.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Consensus Sequence
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology*
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Ethanol / pharmacology
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Gene Expression Regulation, Fungal*
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Glucose / pharmacology*
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Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics*
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Glycerol / pharmacology
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Mutation
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Phosphorylation
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Regulatory Sequences, Nucleic Acid
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Sequence Deletion
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Transcription Factors / genetics
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Transcription Factors / physiology*
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Transcriptional Activation*
Substances
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ABF1 protein, S cerevisiae
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DNA-Binding Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Ethanol
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Glyceraldehyde-3-Phosphate Dehydrogenases
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Glucose
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Glycerol