Abstract
The L1 major capsid protein of human papillomavirus type 16 (HPV-16) was expressed in Sf-21 insect cells with a recombinant baculovirus. Virus-like particles obtained were purified and used to develop an enzyme-linked immunosorbent assay for detection of anti-HPV-16 antibodies in sera from 76 women with evidence of genital HPV infection and 79 controls. HPV-16-infected individuals developed antibodies directed at HPV-16 virions since reactivity against recombinant HPV-16L1 capsids was observed in 50% of them compared with only 6% in the general adult population. However, some cross-reactivities with sera from women infected with others HPV types were observed.
Publication types
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Clinical Trial
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Controlled Clinical Trial
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood*
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Antigens, Viral / genetics
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Baculoviridae / genetics
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Capsid / genetics
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Capsid / immunology
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Cell Line
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Cross Reactions
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Enzyme-Linked Immunosorbent Assay / methods*
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Female
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Humans
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Papillomaviridae / classification
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Papillomaviridae / genetics
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Papillomaviridae / immunology*
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Papillomavirus Infections / diagnosis
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Papillomavirus Infections / immunology
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Spodoptera
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Tumor Virus Infections / diagnosis
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Tumor Virus Infections / immunology
Substances
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Antibodies, Viral
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Antigens, Viral
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Recombinant Proteins