Abstract
Restriction fragment length polymorphism (RFLP) was used to study 75 clinical isolates identified as Mycobacterium avium. Two repetitive insertion sequences, IS1311 and IS900, were used as DNA probes. Although less than 25% of isolates showed RFLP patterns with IS900, all strains gave banding patterns with IS1311. M. avium strains isolated from patients with AIDS exhibited marked polymorphism with both probes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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AIDS-Related Opportunistic Infections / complications
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AIDS-Related Opportunistic Infections / microbiology
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Bacterial Typing Techniques*
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Base Sequence
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DNA Probes / genetics
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DNA Transposable Elements
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DNA, Bacterial / genetics
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Genetic Markers
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Humans
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Molecular Sequence Data
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Mycobacterium avium Complex / classification*
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Mycobacterium avium Complex / genetics*
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Mycobacterium avium-intracellulare Infection / complications
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Mycobacterium avium-intracellulare Infection / microbiology
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length*
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Repetitive Sequences, Nucleic Acid
Substances
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DNA Probes
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DNA Transposable Elements
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DNA, Bacterial
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Genetic Markers