Abstract
Matrix-degrading proteinases secreted by tumor cells play crucial roles in tumor cell invasion and metastasis. Serum-free conditioned media of 7 human gynecological carcinoma cell lines were examined for proteinases and their inhibitors by using gelatin zymography, reverse zymography and immunoblotting. All of three ovarian adenocarcinoma cell lines secreted urokinase-type plasminogen activator. Among them, a mucinous cystadenocarcinoma cell line also secreted tissue-type plasminogen activator, plasmin-like enzyme and trypsinogen. On the other hand, two ovarian undifferentiated carcinoma cell lines mainly secreted glatinase A or B. A choriocarcinoma cell line secreted multiple metalloproteinases in the highest amount, whereas an endometrial adenocarcinoma cell line (HEC-1) derived from an early clinical stage hardly secreted any gelatinolytic enzyme. The five high proteinases producers hardly secreted the corresponding inhibitors, such as tissue inhibitor of metalloproteinases (TIMP)-1, -2 or plasminogen activator inhibitor-1. In contrast to these highly malignant cell lines, a poor proteinase producer, HEC-1, secreted a large amount of TIMPs. Therefore, an enhanced proteolytic tendency appears to be associated with gynecological cancer cells established from highly malignant tumors.
MeSH terms
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Adenocarcinoma, Clear Cell / metabolism
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Carcinoma / metabolism
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Choriocarcinoma / metabolism
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Collagenases / chemistry
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Collagenases / metabolism*
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Culture Media, Serum-Free
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Cystadenocarcinoma, Mucinous / metabolism
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Endometrial Neoplasms / metabolism*
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Female
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Glycoproteins / metabolism
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Humans
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Immunoblotting
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Matrix Metalloproteinase 9
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Matrix Metalloproteinase Inhibitors
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Metalloendopeptidases / antagonists & inhibitors
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Metalloendopeptidases / chemistry
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Metalloendopeptidases / metabolism*
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Molecular Weight
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Neoplasm Proteins / antagonists & inhibitors
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / metabolism*
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Ovarian Neoplasms / metabolism*
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Plasminogen Activator Inhibitor 1 / metabolism
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Plasminogen Activator Inhibitor 2 / metabolism
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Proteins / metabolism
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Serine Endopeptidases / chemistry
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Serine Endopeptidases / metabolism*
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Serine Proteinase Inhibitors / metabolism
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Tissue Inhibitor of Metalloproteinase-2
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Tissue Inhibitor of Metalloproteinases
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Trypsin / metabolism
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Tumor Cells, Cultured
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Urokinase-Type Plasminogen Activator / antagonists & inhibitors
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Urokinase-Type Plasminogen Activator / metabolism*
Substances
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Culture Media, Serum-Free
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Glycoproteins
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Matrix Metalloproteinase Inhibitors
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Neoplasm Proteins
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Plasminogen Activator Inhibitor 1
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Plasminogen Activator Inhibitor 2
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Proteins
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Serine Proteinase Inhibitors
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Tissue Inhibitor of Metalloproteinases
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Tissue Inhibitor of Metalloproteinase-2
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Serine Endopeptidases
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Trypsin
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Urokinase-Type Plasminogen Activator
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Collagenases
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Metalloendopeptidases
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Matrix Metalloproteinase 9