Phase-sensitive two-dimensional experiment on living animals

J Magn Reson B. 1995 Jul;108(1):44-9. doi: 10.1006/jmrb.1995.1100.

Abstract

This report describes the first 1H-1H phased spectrum obtained in vivo from the hind-leg muscles of an intact mouse. TOCSY correlations can follow the complete spin system and give a more detailed graph of each molecule than can COSY. TOCSY is also better suited than COSY for studying long fatty-acid chains. All the peaks are in phase in a TOCSY experiment and the intensities of the cross-correlation peaks are less sensitive to low digitalization in the t1 domain than are those of COSY. The improvement in sensitivity was estimated by measuring the volumes of the cross-correlation peaks in COSY and TOCSY spectra.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carnosine / metabolism
  • Deuterium
  • Fatty Acids / metabolism
  • Hindlimb / metabolism
  • Hydrogen
  • Image Enhancement / methods*
  • Lactates / metabolism
  • Linoleic Acid
  • Linoleic Acids / metabolism
  • Magnetic Resonance Spectroscopy / methods*
  • Mice
  • Muscle, Skeletal / metabolism*
  • Phosphatidylcholines / metabolism
  • Taurine / metabolism
  • Triglycerides / metabolism

Substances

  • Fatty Acids
  • Lactates
  • Linoleic Acids
  • Phosphatidylcholines
  • Triglycerides
  • Taurine
  • Hydrogen
  • Carnosine
  • Linoleic Acid
  • Deuterium