Abstract
Glutamate excretion due to amino acid starvation was investigated in "stringent" and "relaxed" strains of Escherichia coli. The observed excretion process is relA-dependent, carrier-mediated, and glutamate-specific. After induction, excretion was detected within less than 2 min and continued for more than 5 h with a rate of 7-10 nmol (mg dry weight)-1 min-1. Using carbonyl cyanide m-chlorophenylhydrazone or polymyxin B nonapeptide, together with valinomycin, it was shown that glutamate excretion is driven by the membrane potential.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Biological Transport
-
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
-
Escherichia coli / enzymology
-
Escherichia coli / genetics
-
Escherichia coli / metabolism*
-
Genes, Bacterial / genetics
-
Genotype
-
Glutamic Acid / metabolism*
-
Ligases / genetics*
-
Ligases / physiology
-
Membrane Potentials / drug effects
-
Polymyxin B / analogs & derivatives
-
Polymyxin B / pharmacology
-
Pyrophosphatases / genetics*
-
Pyrophosphatases / physiology
-
Valinomycin / pharmacology
Substances
-
Valinomycin
-
Glutamic Acid
-
Carbonyl Cyanide m-Chlorophenyl Hydrazone
-
polymyxin B nonapeptide
-
guanosine-3',5'-bis(diphosphate) 3'-pyrophosphatase
-
Pyrophosphatases
-
Ligases
-
guanosine 3',5'-polyphosphate synthetases
-
Polymyxin B