Abstract
Human leukemic HL60 cells are known to express NAD glycohydrolase (NADase) activity following differentiation into macrophage-like cells by 12-O-tetradecanoylphorbol-13-acetate (TPA) or granulocyte-like cells by retinoic acid (RA) treatment. Recently, it was reported that 46 kDa human leukocyte antigen, CD38, expressed by RA-differentiated HL60 cells contained NADase, ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activities. In the present study we questioned whether the NADase activity found in TPA-differentiated HL60 cells is similar to that found in RA-treated cells. Herein we demonstrate that, unlike what is observed following RA treatment, the NADase activity of TPA differentiated cells associates with a 65 kDa glycosylphosphatidylinositol-anchored NADase.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-ribosyl Cyclase
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ADP-ribosyl Cyclase 1
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Antigens, CD*
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Antigens, Differentiation / analysis
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Antigens, Differentiation / metabolism
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Cell Differentiation / drug effects
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Cell Membrane / enzymology
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Glycosylphosphatidylinositols / metabolism*
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Humans
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Leukemia, Promyelocytic, Acute / enzymology*
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Membrane Glycoproteins
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N-Glycosyl Hydrolases / analysis
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N-Glycosyl Hydrolases / metabolism
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NAD+ Nucleosidase / metabolism*
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Tetradecanoylphorbol Acetate / pharmacology*
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Tretinoin / pharmacology
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Tumor Cells, Cultured
Substances
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Antigens, CD
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Antigens, Differentiation
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Glycosylphosphatidylinositols
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Membrane Glycoproteins
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Tretinoin
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N-Glycosyl Hydrolases
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ADP-ribosyl Cyclase
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CD38 protein, human
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NAD+ Nucleosidase
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ADP-ribosyl Cyclase 1
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Tetradecanoylphorbol Acetate