A Drosophila CREB/CREM homolog encodes multiple isoforms, including a cyclic AMP-dependent protein kinase-responsive transcriptional activator and antagonist

Mol Cell Biol. 1995 Sep;15(9):5123-30. doi: 10.1128/MCB.15.9.5123.

Abstract

We have characterized a Drosophila gene that is a highly conserved homolog of the mammalian cyclic AMP (cAMP)-responsive transcription factors CREB and CREM. Uniquely among Drosophila genes characterized to date, it codes for a cAMP-responsive transcriptional activator. An alternatively spliced product of the same gene is a specific antagonist of cAMP-inducible transcription. Analysis of the splicing pattern of the gene suggests that the gene may be the predecessor of the mammalian CREB and CREM genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Blotting, Northern
  • Cloning, Molecular
  • Conserved Sequence
  • Cyclic AMP Response Element Modulator
  • Cyclic AMP Response Element-Binding Protein / genetics*
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • DNA-Binding Proteins / genetics
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Genes, Insect / genetics*
  • Head / embryology
  • In Situ Hybridization
  • Molecular Sequence Data
  • Protein Binding
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transcription, Genetic

Substances

  • CrebB protein, Drosophila
  • Cyclic AMP Response Element-Binding Protein
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Repressor Proteins
  • Trans-Activators
  • Cyclic AMP Response Element Modulator

Associated data

  • GENBANK/S79274