Abstract
We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Agrobacterium tumefaciens / genetics*
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Amino Acid Oxidoreductases / genetics
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Base Sequence
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Cloning, Molecular / methods
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DNA, Bacterial / genetics*
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Genetic Vectors*
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Kanamycin Kinase
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Molecular Sequence Data
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Plants / genetics*
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Plants, Genetically Modified
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Plasmids
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Replication Origin
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Transformation, Genetic*
Substances
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DNA, Bacterial
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Amino Acid Oxidoreductases
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nopaline synthase
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Phosphotransferases (Alcohol Group Acceptor)
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Kanamycin Kinase