Expression of the death-associated gene RP-8 in granule cell neurons undergoing postnatal cell death in the cerebellum of weaver mice

Brain Res Dev Brain Res. 1995 May 26;86(1-2):35-47. doi: 10.1016/0165-3806(95)00004-w.

Abstract

RP-8 is one of several mRNAs elevated during apoptosis in immature thymocytes. We used in situ hybridization to look for RP-8 mRNA in the cerebella of weaver mice. In the weaver mouse cerebellar granule cells fail to differentiate and instead die during the first two weeks of postnatal development [30]. We demonstrate that the dying cells exhibit DNA degradation, a characteristic feature of apoptosis. RP-8 is expressed in the weaver cerebellum during the period of granule cell death, and is limited to cells in the same region where apoptotic granule cells are located. Thus, RP-8 expression, first associated with PCD in thymocytes, correlates with granule cell death in the weaver cerebellum.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Apoptosis / physiology*
  • Base Sequence
  • Cerebellar Cortex / cytology
  • Cerebellar Cortex / metabolism
  • Cerebellum / cytology
  • Cerebellum / physiology*
  • Gene Expression*
  • Genes
  • In Situ Hybridization
  • Mice
  • Mice, Neurologic Mutants
  • Molecular Sequence Data
  • Neurons / physiology*
  • Oligonucleotide Probes / genetics
  • RNA, Messenger / metabolism

Substances

  • Oligonucleotide Probes
  • RNA, Messenger