Abstract
The labelling of ex-vivo activated macrophages with doses of 111In-oxine sufficient for gamma camera imaging does not damage cell viability or the functional competence of cells (secretion of tumour necrosis factor-alpha and interleukin-6). The mean labelling efficiency was about 84%. Release of 111In equivalent to 15% occurred over 24 h under cell culture conditions, indicating good stability of the radioactive cell label. 111In-oxine-labelled macrophages are a suitable tool for biokinetic studies of adoptive immunotherapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Survival
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Cytotoxicity, Immunologic
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Humans
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Immunotherapy, Adoptive
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In Vitro Techniques
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Indium Radioisotopes* / pharmacokinetics
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Macrophage Activation
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Macrophages / cytology
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Macrophages / immunology*
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Macrophages / metabolism
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Monocytes / immunology
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Neoplasms / diagnostic imaging*
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Neoplasms / immunology*
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Neoplasms / therapy
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Organometallic Compounds* / pharmacokinetics
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Oxyquinoline / analogs & derivatives*
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Oxyquinoline / pharmacokinetics
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Radionuclide Imaging
Substances
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Indium Radioisotopes
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Organometallic Compounds
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indium oxine
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Oxyquinoline