Abstract
An enzyme-linked immunoassay for human C4b-binding protein (concentration range 25-400 ng/ml) was developed using two monoclonal antibodies; the intra- and interassay coefficients of variation were less than 7.2%. In 50 normal subjects, 20 patients with liver cirrhosis and 34 full-term newborns, the plasma levels of C4b-binding protein were very similar to those measured by Laurell electroimmunoassay; in 24 patients with elevated erythrocyte sedimentation rates, levels measured by enzyme-linked immunoassay were higher then those measured by the Laurell method (270 +/- 70% vs. 223 +/- 67%). In these patients crossed immunoelectrophoresis showed a pattern different from that of normal individuals, which may explain the lower values found with the Laurell method.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Animals
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Antibodies, Monoclonal
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Binding, Competitive
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Blood Sedimentation
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Carrier Proteins / blood*
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Carrier Proteins / immunology
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Complement C4b / metabolism*
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Complement Inactivator Proteins*
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Evaluation Studies as Topic
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Female
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Glycoproteins*
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Humans
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Immunoassay / methods*
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Immunoassay / statistics & numerical data
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Immunoelectrophoresis, Two-Dimensional / methods
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Immunoelectrophoresis, Two-Dimensional / statistics & numerical data
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Immunoenzyme Techniques
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In Vitro Techniques
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Infant, Newborn
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Inflammation / blood
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Liver Cirrhosis / blood
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Male
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Mice
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Protein S / metabolism
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Reference Values
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Antibodies, Monoclonal
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Carrier Proteins
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Complement Inactivator Proteins
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Glycoproteins
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Protein S
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Complement C4b