Molecular cloning of cDNAs encoding human GLEPP1, a membrane protein tyrosine phosphatase: characterization of the GLEPP1 protein distribution in human kidney and assignment of the GLEPP1 gene to human chromosome 12p12-p13

Genomics. 1995 May 1;27(1):174-81. doi: 10.1006/geno.1995.1021.

Abstract

Human glomerular epithelial protein 1 (GLEPP1), a receptor-like membrane protein tyrosine phosphatase (PTPase), was cloned and sequenced from a human renal cortical cDNA library. The human nucleotide and derived amino acid sequences were, respectively, 90 and 97% identical to those of rabbit. Human GLEPP1 is predicted to contain 1188 amino acids. The predicted mature protein is 1159 amino acids long and contains a large extracellular domain, a single transmembrane domain, and a single intracellular PTPase domain. Monoclonal and polyclonal antibodies raised against a human GLEPP1 fusion protein recognized a protein with distribution restricted to the glomerulus in human kidney and with an apparent molecular weight of approximately 200 kDa. The GLEPP1 gene was assigned to human chromosome 12p12-p13 by fluorescence in situ hybridization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Base Sequence
  • Cell Size
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12*
  • DNA, Complementary / genetics
  • Genes*
  • Humans
  • Kidney Glomerulus / cytology
  • Kidney Glomerulus / enzymology*
  • Membrane Proteins / analysis
  • Membrane Proteins / genetics*
  • Membrane Proteins / immunology
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Molecular Weight
  • Protein Tyrosine Phosphatases / analysis
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / immunology
  • Protein Tyrosine Phosphatases / physiology
  • Rabbits
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3
  • Receptors, Cell Surface / analysis
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / immunology
  • Receptors, Cell Surface / physiology
  • Recombinant Fusion Proteins / immunology
  • Sequence Homology, Amino Acid

Substances

  • Antibodies, Monoclonal
  • DNA, Complementary
  • Membrane Proteins
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • PTPRO protein, human
  • Protein Tyrosine Phosphatases
  • Receptor-Like Protein Tyrosine Phosphatases, Class 3

Associated data

  • GENBANK/U20489