Abstract
The human immunodeficiency virus type 1 strain MN (HIV-1MN) principal neutralizing determinant (PND, V3 loop) was introduced into infectious molecular clones HIV-2KR and simian immunodeficiency virus mm239 (SIVmm239) by hybridization PCR, replacing the corresponding HIV-2 or SIV envelope cysteine loops with the HIV-1 coding sequence. The HIV-2 chimera (HIV-2KR-MNV3) was found to be capable of infecting a number of T-cell lymphoblastic cell lines as well as primary peripheral blood mononuclear cells. In contrast, the SIV chimera (SIV239MNV3) was not replication competent. Envelope produced by HIV-2KR-MNV3 but not the parental HIV-2KR was recognized by V3-specific and HIV-1-specific polyclonal antisera in radioimmunoprecipitation assays. HIV-2-specific antisera recognized both the chimeric and parental virus but not HIV-1MN. The chimeric HIV-2KR-MNV3 virus proved to be exquisitely susceptible to neutralization by HIV-1-specific and V3-specific antisera, suggesting the potential for use in animal models designed to test HIV-1 vaccine candidates which target the PND.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Cell Line
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Chimera
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Cysteine
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DNA Primers
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Gene Products, env / biosynthesis*
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Gene Products, env / genetics
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Gene Products, env / immunology*
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HIV-1 / genetics
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HIV-1 / physiology*
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HIV-2 / genetics
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HIV-2 / pathogenicity
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HIV-2 / physiology*
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Humans
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Kinetics
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Molecular Sequence Data
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Mutagenesis
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Polymerase Chain Reaction
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Radioimmunoprecipitation Assay / methods
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Simian Immunodeficiency Virus / physiology
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T-Lymphocytes / immunology
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T-Lymphocytes / virology*
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Tumor Cells, Cultured
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Virus Replication*
Substances
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DNA Primers
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Gene Products, env
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Cysteine
Associated data
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GENBANK/M33262
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GENBANK/M61062
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GENBANK/M61063
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GENBANK/M61064
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GENBANK/M61065
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GENBANK/M61066
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GENBANK/M61067
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GENBANK/M61068
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GENBANK/M61069
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GENBANK/M61070
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GENBANK/M61071
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GENBANK/M61072
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GENBANK/M61073
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GENBANK/M61074
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GENBANK/M61075
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GENBANK/M61076
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GENBANK/M61077
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GENBANK/M61078
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GENBANK/M61079
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GENBANK/M61080
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GENBANK/M61081
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GENBANK/M61082
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GENBANK/M61083
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GENBANK/M61084
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GENBANK/M61085
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GENBANK/M61086
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GENBANK/M61087
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GENBANK/M61088
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GENBANK/M61089
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GENBANK/U22047