Abstract
Vaccinia virus with the E3L gene deleted was able to replicate in RK-13 but not HeLa cells. This host range phenotype could be complemented by an E3L gene expressed transiently from a plasmid. Analysis of mutants of E3L indicates that the ability to complement deletion of E3L correlates with the ability of mutated proteins to bind double-stranded RNA but not with their ability to migrate to the nucleus.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cell Line
-
Fluorescent Antibody Technique
-
Gene Deletion*
-
Genes, Viral*
-
HeLa Cells
-
Humans
-
RNA-Binding Proteins / analysis
-
RNA-Binding Proteins / biosynthesis
-
RNA-Binding Proteins / genetics*
-
Rats
-
Vaccinia virus / genetics
-
Vaccinia virus / physiology*
-
Viral Proteins / analysis
-
Viral Proteins / biosynthesis*
-
Viral Proteins / genetics*
-
Viral Proteins / isolation & purification
-
Virus Replication*
Substances
-
E3L protein, Vaccinia virus
-
RNA-Binding Proteins
-
Viral Proteins