Abstract
We have identified a target antigen in autoimmune cerebellar degeneration, beta-NAP, that is closely related to the beta-adaptin and beta-COP coat proteins. Beta-NAP is a nonclathrin-associated phosphoprotein expressed exclusively in neurons, from E12 through adulthood. Beta-NAP is present in the neuronal soma and nerve terminal as soluble and membrane-bound pools and is associated with a discrete set of nerve-terminal vesicles. These results establish beta-NAP as a neuron-specific vesicle coat protein. We propose a model in which beta-NAP mediates vesicle transport between the soma and the axon terminus and suggest that beta-NAP may represent a general class of coat proteins that mediates apical transport in polarized cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Protein Complex 3 / analysis
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Adaptor Protein Complex 3 / genetics*
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Adaptor Protein Complex beta Subunits / analysis
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Adaptor Protein Complex beta Subunits / genetics*
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Animals
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Antigens / physiology
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Capsid
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Cerebellar Diseases / immunology*
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Cerebellum / chemistry
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Cerebellum / embryology
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Cerebellum / pathology
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Fetus / physiology
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Gene Expression / physiology
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Immunohistochemistry
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Membrane Proteins / analysis
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Membrane Proteins / genetics*
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Mice
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Microscopy, Electron
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Molecular Sequence Data
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / genetics*
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Neurons / chemistry*
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Phosphoproteins / analysis
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Phosphoproteins / genetics*
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Presynaptic Terminals / chemistry
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RNA, Messenger / analysis
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Sequence Homology, Amino Acid
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Synaptic Vesicles / chemistry
Substances
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AP-3b2 adaptor protein, mouse
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AP3B2 protein, human
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Adaptor Protein Complex 3
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Adaptor Protein Complex beta Subunits
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Antigens
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Membrane Proteins
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Nerve Tissue Proteins
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Phosphoproteins
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RNA, Messenger