Abstract
The multidrug resistance (mdr1) gene product, P-glycoprotein, is responsible for the ATP-dependent extrusion of a variety of compounds, including chemotherapeutic drugs, from cells. The data presented here show that cells with increased levels of the P-glycoprotein release ATP to the medium in proportion to the concentration of the protein in their plasma membrane. Furthermore, measurements of whole-cell and single-channel currents with patch-clamp electrodes indicate that the P-glycoprotein serves as an ATP-conducting channel in the plasma membrane. These findings suggest an unusual role for the P-glycoprotein.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Adenosine Triphosphate / metabolism*
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Amino Acid Sequence
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Animals
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Antibodies
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CHO Cells
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Cell Membrane / physiology
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Cricetinae
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Drug Resistance / genetics*
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Humans
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Ion Channels / physiology*
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Lung Neoplasms
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Mice
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Molecular Sequence Data
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Peptides / immunology
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Recombinant Fusion Proteins / metabolism
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Transfection
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Tumor Cells, Cultured
Substances
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Antibodies
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Ion Channels
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Membrane Glycoproteins
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Peptides
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Recombinant Fusion Proteins
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Adenosine Triphosphate