Cytokine production by peripheral blood monocytes and T cells during haemopoietic recovery after intensive chemotherapy

Br J Haematol. 1993 Jan;83(1):21-7. doi: 10.1111/j.1365-2141.1993.tb04625.x.

Abstract

We studied the production of cytokines by peripheral blood monocytes and T cells during the period of haematological recovery following intensive chemotherapy. Twelve adults with haematological malignancies received consolidation chemotherapy of complete remission. Monocytes and T cells were collected during the phase of recovery from intensive chemotherapy, and were incubated for 24 h in a culture medium with 10% FCS. Concentrations of cytokines in the culture supernatant were measured with an enzyme-linked immunosorbent assay. During the recovery phase, concentrations of IL-6, G-CSF and IL-1 beta in the culture supernatant of the collected monocytes significantly exceeded those of the monocytes obtained from normal healthy subjects. Similarly, the concentrations of GM-CSF and IFN-gamma in the supernatant of recovery phase T cells significantly exceeded those of normal T cells. Plasma levels of these cytokines were also elevated. These data suggest that the monocytes and T cells may be activated in vivo to produce haemopoietic cytokines during haematological recovery, and that, during haematological recovery, the monocytes and T cells may be actively involved in the induction of haematopoiesis following the myelosuppression induced by chemotherapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Cytokines / biosynthesis*
  • Female
  • Granulocyte Colony-Stimulating Factor / biosynthesis
  • Humans
  • Interleukin-1 / biosynthesis
  • Interleukin-6 / biosynthesis
  • Leukemia, Myeloid / blood*
  • Leukemia, Myeloid / drug therapy
  • Male
  • Middle Aged
  • Monocytes / metabolism*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / blood*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / drug therapy
  • T-Lymphocytes / metabolism

Substances

  • Cytokines
  • Interleukin-1
  • Interleukin-6
  • Granulocyte Colony-Stimulating Factor