Abstract
We have analyzed the regulation of fibroblast growth factor receptors (FGFRs) during retinoic acid (RA) induced differentiation of Tera-2 human embryonal carcinoma cells. Undifferentiated Tera-2 cells expressed mRNAs for all four known FGFRs. Their differentiation led to loss of FGFR-4 mRNA expression and mRNA levels for FGFR-2 and FGFR-3 were considerably downregulated, whereas the mRNA levels for FGFR-1 remained unaltered. A substantial decrease in binding of K-FGF was found to occur upon RA-induced differentiation of the cells. In undifferentiated Tera-2 cells FGF stimulation caused an increase of c-fos mRNA, and c-jun mRNAs, but no increase of junB mRNA, whereas in the differentiated cells, FGFs strongly stimulated the expression of all three genes. Thus differentiation of the Tera-2 cells leads to marked changes in FGFR gene expression as well as to complex alterations in their responses to exogenous FGFs.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Differentiation / drug effects
-
Cell Differentiation / physiology*
-
Clone Cells
-
Fibroblast Growth Factor 1 / metabolism
-
Fibroblast Growth Factor 1 / pharmacology*
-
Fibroblast Growth Factor 2 / metabolism
-
Fibroblast Growth Factor 2 / pharmacology*
-
Genes, fos / drug effects
-
Genes, jun / drug effects
-
Humans
-
Poly A / genetics
-
Poly A / isolation & purification
-
RNA / genetics
-
RNA / isolation & purification
-
RNA, Messenger / metabolism
-
Receptors, Fibroblast Growth Factor / genetics*
-
Receptors, Fibroblast Growth Factor / isolation & purification
-
Receptors, Fibroblast Growth Factor / metabolism
-
Signal Transduction / drug effects*
-
Teratoma
-
Thymidine / metabolism
-
Tretinoin / pharmacology*
-
Tritium
-
Tumor Cells, Cultured
Substances
-
RNA, Messenger
-
Receptors, Fibroblast Growth Factor
-
Tritium
-
Fibroblast Growth Factor 2
-
Fibroblast Growth Factor 1
-
Poly A
-
Tretinoin
-
RNA
-
Thymidine