A sequential study on the use of the cytokinesis-block micronucleus method in mouse splenocytes

Mutat Res. 1993 Apr;301(4):223-7. doi: 10.1016/0165-7992(93)90061-y.

Abstract

Several known clastogens and mutagens have been tested for their ability to induce micronuclei (MN) using the cytokinesis-block method in mouse splenocytes. The chemicals were harringtonine, cisplatin, cytosine arabinoside, vincristine sulfate, colchicine, potassium chromate, methyl methanesulfonate and 2-acetylaminofluorene. All chemicals tested induced a dose-dependent increase in MN and a delay in cell-cycle progression. The results suggest that the cytokinesis-block micronucleus method in mouse splenocytes is reliable, economical and sensitive enough for detecting mutagenic agents in vivo and in vitro.

MeSH terms

  • 2-Acetylaminofluorene / toxicity
  • Animals
  • Cell Cycle / drug effects
  • Cell Division / drug effects*
  • Chromates / toxicity
  • Cisplatin / toxicity
  • Colchicine / toxicity
  • Cytarabine / toxicity
  • Harringtonines / toxicity
  • Male
  • Methyl Methanesulfonate / toxicity
  • Mice
  • Mice, Inbred C57BL
  • Micronucleus Tests / methods*
  • Mutagens / toxicity*
  • Potassium Compounds*
  • Spleen / cytology
  • Spleen / drug effects*
  • Vincristine / toxicity

Substances

  • Chromates
  • Harringtonines
  • Mutagens
  • Potassium Compounds
  • Cytarabine
  • harringtonin
  • Vincristine
  • potassium chromate(VI)
  • 2-Acetylaminofluorene
  • Methyl Methanesulfonate
  • Cisplatin
  • Colchicine