Abstract
Putative hepatitis C virus core sequence was amplified from a serum sample positive for anti-C-100-3 and expressed in Escherichia coli. Approximately 62 kDa fusion protein with maltose binding protein containing 20 kDa hepatitis C core protein was obtained. The antibody to this protein was detected in 53 of 54 (98%) sera from hepatitis C virus ribonucleic acid-positive patients including 40 sera positive for anti-C-100-3 and 13 sera negative for anti-C-100-3. The antibody was also detected in all of 12 patients with acute hepatitis C showing the earlier detectability of the antibody than anti-C-100-3. Thus, the protein expressed from the amplified hepatitis C core sequence by the polymerase chain reaction would be useful for the diagnosis of hepatitis C.
MeSH terms
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ATP-Binding Cassette Transporters*
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Acute Disease
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Bacterial Proteins / immunology*
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Base Sequence
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Blotting, Western / methods
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Carrier Proteins / immunology*
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Chronic Disease
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Escherichia coli Proteins*
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Escherichia coli*
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Female
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Hepacivirus / immunology*
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Hepatitis Antibodies / blood*
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Hepatitis C / diagnosis
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Hepatitis C Antibodies
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Humans
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Maltose-Binding Proteins
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Middle Aged
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Molecular Sequence Data
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Molecular Weight
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Monosaccharide Transport Proteins*
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Polymerase Chain Reaction / methods
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RNA, Viral / blood
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Viral Core Proteins / immunology*
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Viral Fusion Proteins / immunology*
Substances
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ATP-Binding Cassette Transporters
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Bacterial Proteins
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Carrier Proteins
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Escherichia coli Proteins
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Hepatitis Antibodies
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Hepatitis C Antibodies
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Maltose-Binding Proteins
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Monosaccharide Transport Proteins
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RNA, Viral
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Viral Core Proteins
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Viral Fusion Proteins
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maltose transport system, E coli